Cell Culture Lab

Cell culture facility refers to methods about removal of cells from an animal/plant and subsequent growing in controlled artificial environment for basic and applied research. The essential requirement of a cell culture laboratory is maintaining an aseptic work area that is restricted to cell culture work.

Major Equipment Facilities

• CO2 Incubator (Make:Eppendorf, Model: CellXpert C170)
• Biological Safety Cabinet Class II (Make: Esco, Model: Airstream® AC2-4S9-NS)
• Inverted Fluorescence Microscopes (Make: Leica, Model: DMi8)
• Benchtop Refrigerated Centrifuge (Make: Eppendorf, Model: 5804 R & 5424 R)
• Microplate Reader (Make: Thermo Scientific, Model: Multiskan FC)
• Automated Cell Counter (Make: Thermo Scientific Invitrogen, Model: Countess II FL)
• Microvolume UV-Vis Spectrophotometer (Make: Thermo Scientific, Model: NanoDrop One)
• Inverted Microscope (Make: Olympus, Model: CKX53)
• Ultra-Low Temperature Freezer (Make: Eppendorf, Model: U410 Premium)
• Deep Freezer (Make: Eltek, Model: EDF-300)
• Upright Automated Autoclave (Make: Hirayama, Model: HICLAVE HV-50 II)

Key Features

• CO2 Incubator: High volume (170 L ) with standard display, seamless chamber, fan-less design, 140°C High Temperature Disinfection.
• Biological Safety Cabinet: Stainless steel interior, single-piece tray, sidewalls, microprocessor controller, low noise, 4 ft width.
• Inverted Fluorescence Microscopes: Transparent, Fluorescence, Phase Contrast, LASX software, DAPI, FITC, RHOD filters.
• Benchtop Refrigerated Centrifuge: Up to 20,913 × g, temp -9°C to 40°C, swing-bucket and fixed-angle rotors for tubes 0.2–250 mL, plate rotor for MTP/PCR/Cell culture/Deepwell Plates.
• Microplate Reader: 340–850 nm, 96-well plate reading <7s, linear shaking, incubation up to 50°C, SkanIt software.
• Automated Cell Counter: Cell viability, cycle phase, apoptosis, fluorescent protein expression, <10s per sample, autofocusing.
• Microvolume UV-Vis Spectrophotometer: 190–850 nm, DNA/RNA/Protein analysis with 1–2 µL, full-spectral data acquisition.
• Inverted Microscope: High contrast, live cell observation, sampling, 10x, 20x, 40x, universal holder for dishes, microplates, flasks.
• Ultra-Low Temperature Freezer: 410L, temp -50°C to -86°C, 5 compartments, insulated inner door, 4 adjustable shelves, 24,000 sample capacity.
• Deep Freezer: 300L, temp -25°C, microprocessor control, CFC-free gas, high density PUF insulation, LCD display, audio/visual alarm, stainless steel inner.
• Automated Autoclave: 50L, color touch panel, electro-mechanical lid lock, dual interlock, air removal time setting, sterilizes liquids at high temp and pressure.